The paper presents a theoretical and literature review of research on different procedures for the chitin separation from food waste shells and the impact of the type of process on the properties of chitin/chitosan, the results are take over from references presented in the Methodology. [17, 18] The chemical extraction process of chitin from the food waste shells is very efficient and economically justified. Its disadvantage is production of toxic byproducts at the end of the process, which must be treated before disposal, which makes the process become more expensive. It can be seen from the literature that the chitin and its chitosan derivatives thus isolated have very good properties and are stable and suitable for use in medicine, where the requirements for the stability and health safety of biopolymers are particularly high. Research on the separation of chitin from lobster shells by biochemical processes using several different microbial cultures (Bacillus megaterium NH21, Serratia marcescens db11 Lactobacillus plantarum i Serratia marcescens db11) as a biocatalyst was also performed. Extraction processes were carried out with each separate microbial culture and with their mixtures. The process of deproteinization and demineralization has been particularly efficient with the application of these two cultures in combination; Serratia marcescens db11 and Lactobacillus plantarum. The results indicate that CaCO3 (89,59%) and protein (87,19%) were removed and the chitin yield was 82,56% from lobster shells.