Polyphenol compounds are secondary metabolites contained in plants. Comprise a large number of compounds having aromatic rings with one or more hydroxyl groups. Polyphenols have an effect on human health: antibacterial, anti-inflammatory, antioxidant, anti-cancer. The biological function of polyphenols attributed to their excellent antioxidant and antiradical activities. Grapes, the main place where takes place the biosynthesis of secondary metabolites that are of great importance for the quality of wine. In grapes, except phenolic compounds seeds, the largest share of phenolic compounds is in the skin of the berries. The subjects of our research were polyphenols: anthocyanins, flavonols, and flavan-3-ol in the skin of grapes Regent. Polyphenols from grape skins, we extracted microwave-assisted extraction. The obtained extracts were analysed by high performance liquid chromatography with a photodiode array detector (DAD) and fluorescence detector (FLD). For chromatographic separation was used column Luna Phenyl-Hexyl Phenomenex, USA). Column dimensions were 250 mm x 4.6 mm and the grain size is 5 microns, with a Phenyl precolumn (4.0 * 3.0). The mobile phase consisted of: A water / phosphoric acid (99,5 / 0,5 / v / v) and B is acetonitrile / water / phosphoric acid (50 / 49,5 / 0,5 / v / v / v). The aim of this study was extract polyphenolic compounds from the skins of grapes varieties Regent and optimize the microwave extraction method. Analyses show that microwave assisted extraction is a very effective methods of extracting anthocyanins from 50 % ethanol at T = 40 °C to t = 10 minutes; flavonols with 60 % acetonitrile at T = 40 °C for t = 30 min; flavan-3-ol with 20 % acetonitrile at T = 60 °C for t = 5 minutes. In the process of optimization of the extraction of compounds is performed with 60 % EtOH at T = 40 °C for t = 5 minutes. RSD values newoptimized methods are very low (< 2 % of flavonols and anthocyanins, and about 9 % of flavan-3-ole), which indicates that the method is accurate and precise. This scientific research can be used for better use grapes as a basis for obtaining the flavonoids for commercial purposes.