During the 20th and early 21st century, there have been significant scientific development in the field of nanotechnology and the research on nanostructured materials, including mesoporous materials and mesoporous silica. Mesoporous materials have a porous structure and a high specific surface area that enables their wide application in various fields of science and technology. They are classified based on their pore size and structure. Mesoporous SiO2 particles of various structures, morphologies and thus properties, can be prepared by altering synthesis conditions. Mesoporous materials represent an important material with significant potential for application in various fields of science and technology, rendering this field interesting for further research. The important application of mesoporous silica is in enzyme immobilization where is used as carrier. Immobilized enzymes are widely used in industrial and biotechnological processes, because they retain their catalytic activity and can be used continuously and repeatedly. The aim of this study was to synthesize and characterize mesoporous silica particles with different pore sizes by adjusting the ratio of components involved in the formation of the mesoporous structure.