The apple is one of the most widespread fruit species in the world, used both for fresh consumption and for the production of various processed products. The processing of apples produces a significant amount of byproduct known as apple pomace, which has potential nutritional value due to the presence of many nutrients, including polyphenols, known for their antioxidant properties. Due to its nutritional composition, apple pomace is used in numerous industries, from food to pharmaceuticals. For this reason, the aim of this study is to determine the application of the beneficial polyphenols in apple pomace in daily life and to perform a qualitative analysis of the polyphenolic compounds for further use. The study was carried out on four different apple varieties and the amounts of nutrients in apple pomace were determined by HPLC analysis of the individual phenolic compounds as well as the total phenolic, flavonoid and non-flavonoid compounds. These results were also confirmed by antioxidant tests using the ABTS, FRAP and DPPH methods. The study shows the great potential of apple pomace as a source of natural polyphenols with applications in various industries. The study contributes to the understanding of the nutritional value of apple pomace and its potential use in the production of food supplements and other products. The research results can serve as a basis for further studies and commercial applications of polyphenols from apple pomace.