Increasing need for the replacement of a specific tissue or organ, with a smaller number of donors, entail a new sphere of interest through tissue engineering and tissue development in vitro. Such an undertaking is based on the grafting of a cell on an extracellular matrix carrier made of biomaterials with pronounced biocompatibility. In doing so, a three-dimensional complex tissue is created, which is then transplanted. In order for the extracellular matrix to be isolated, become a carrier for cells and retain its structure, it is necessary to go through the decellularization process. In this process, the tissue is subjected to physical, chemical and enzymatic procedures. In this work, decellularization of porcine liver was carried out by a chemical method using 1% solutions of detergents sodium lauryl sulfate (SDS), sodium laureth sulfate (SLES), Triton-X 100 and Tween 20. After the decellularization, the obtained samples were tested using quantification methods, in order to measure the presence of DNA, collagen and GAG for the detection of residual biological material. In addition to various methods of quantification, the tissue structure was analyzed using a light microscope and a stereomicroscope. The research results showed variable effectiveness of detergents such as SDS, SLES, Triton-X 100, and Tween 20 in the decellularization process of porcine liver. Quantification of DNA, collagen, and glycosaminoglycan (GAG) content in decellularized tissue samples revealed the different abilities of detergents in preserving these key components. Tissue micrographs further illustrated damages and ruptures in the extracellular matrix structure, with some detergents causing greater damage than others. These findings emphasize the need for careful selection of detergents and optimization of the decellularization process to minimize tissue structure damage and ensure their biocompatibility for future applications.