Physico-chemical and morphological characterizations of mesoporous nanoparticles were conducted within the framework of this research using several techniques: UV/Vis spectrophotometry, infrared spectroscopy including Fourier transform, scaning electron microscope (SEM) and dynamic light scattering (DLS). The research was performed using three different flavonoids (quercetin, myricetin and myricitrin) that were dissolved in EtOH/H2O solution and incorporated in Fe3O4 nanoparticles. The quantity of incorporated flavonoids in nanoparticles was determined by spectrophotometric measurment of supernatant with UV/Vis spectrometer. After incorporation of flavonoid within nanoparticles, their release profiles were followed by during the experiment the focus was on releasing the flavonoids from the nanoparticles at the standard conditions and in permanent and oscillating magnetic field using UV/Vis spectrophotometry . The characterisation of the empty and flavonoid loaded magnetic nanoparticles as well as The morphology and distribution of the nanoparticles were carried out by Fourier transform infrared spectroscopy (FTIR) analysis, SEM and DLS measurement. SEM confirmed that the change occured at the surface roughness of the magnetic nanoparticles after the incorporation of flavonoids. Due to low concentration of incorporated flavonoids it was not possible to quantitatively determine the concentration and nature of the link between the nanoparticles and flavonoids by Fourier transform infrared spectroscopy (FTIR) analysis. In the research the stability of the empty Fe3O4 nanoparticles and nanoparticles loaded with flavonoid dissolved in PBS buffer was examined by the DLS method during 96 hours.