The aim of this work is to investigate and compare the capabilities of specific fingerprint powders for latent fingerprint visualization on six different substrates. The time from fingerprint deposition to the moment of visualization was also taken into account. The studied powders were: Instant Black, Supranano Black, Fluorescent Magnetic Red and Supranano Fluorecent Red Magnetic. First of all, characterization of the choosen powders has been carried out with SEM and EDX analysis. The next step of research started with the deposition of fingerprints, which are then periodically developed after 0, 5, 10, 15 and 20 days by using mentioned powders. The investigation on the following substrates: transparent plastic, black plastic, glass, lacquered wood, stainless steel and laminated paper was done. The developed fingerprints are then recorded with a professional camera (Nikon D7100), afterwards the images were compared considering the surface and the moment of development. To visualized latent fingerprints certain marks (usable - 2, usable with a correction - 1, unusable - 0), were ascribed. The marks are then summarized and graphically expressed as a function of the type of substrate and the type of applied powders. Knowledge about influence of dimensions, shape and particle size distribution, degree of aglomeration and elemental composition of the powders on the fingerprint quality was obtained. Results showed that none of the investigated powders is universal and does not produce prints of good quality on very surfaces. The best resolution gives supranano black and supranano red fluorescent magnetic powder. Reasons for better quality of developed prints with nano powders may be, inter alia, due to a lesser dimension of the particles, more regularly shaped particles and a smaller particle size distribution as shown in the SEM micrographs.