Chemotherapeutic agents are medicines of natural or synthetic origin and they are used for the prevention and treatment of diseases in humans and animals. Due to increased pharmaceutical consumption they are present in the environment, water, soil and sediment in uncontrollable quantities. There is no sufficient data to predict their behavior and the impact on the environment, therefore they are considered as "emerging contaminants". Pharmaceuticals investigated in this study belong to the various groups of pharmaceuticals by their therapeutic effects: antibiotics (nitrofurantoin, cefdinir), antiemetics (metoclopramide), anthelmintics or antiparasitics (albendazole, febantel, praziquantel) and chemotherapeutic agent (hydroxychloroquine). The aim of this study was to optimize the conditions for extraction of seven chemotherapeutic agents simultaneously by agitation from sediment. Parameters that were optimized are type of solvent, influence of the pH value, mass of the sample and time of the extraction. Extracts were analyzed by high-performance liquid chromatography with diode array detector (HPLC-DAD). The best results were obtained with solvent systems methanol:hexane and acetone:methanol with 0,7 grams of the sediment sample. However, this method is not recommended for routine analysis because recoveries of the analyzed pharmaceuticals were not higher than 50 %.