The pharmaceuticals are of particular concern due to their ubiquity in the aquatic environment and their health effects. Since primary and secondary wastewater treatment (WWT) technologies are not specifically designed to remove some of contaminants of emerging concern (CECs) and their metabolites, pharmaceuticals are detected in different environmental matrices worldwide; in WWT plant effluents, surface, ground and drinking waters. Current Directive 2013/39 / EC included the pharmaceutical 17β-estradiol (E2) in the "watch" list. 17β-Estradiol is a female sex hormone and steroid drug which can have adverse effects on various organisms at very low concentrations. Because WWT systems are not fully effective in removing E2, in order to solve this problem it is necessary to develop new technologies that can completely remove or destroy its biological activity. The aim of this study was to evaluate the possibilities of advanced oxidation processes, especially photooxidative process UV-C/H2O2 model for treating model wastewater containing E2 dissolved in aqueous acetonitrile. The influence of key operating parameters of applied photooxidative processes (pH and concentration of oxidant) were investigated using the combination of full factorial experimental design. The influence of pH and concentration of H2O2 on the kinetics of E2 degradation were experimentally evaluated. Degradation of E2 obeys first-order kinetics, which was accurately modeled by the developed RSM model which takes into account the pH and concentration of H2O2. Observed reaction rates were in the range from 8.066 × 10-4 s-1 to 40.74 × 10-4 s-1. The optimal conditions yielding the highest degradation rate were at pH 7 and [H2O2] = 50 mM.