This thesis first provides an overview of research in the field of application in gamification of e-learning. A survey was conducted among teachers who use e-learning to work with students, followed by pilot research, and the first and second main research. The research falls into the field of social sciences, where it is verified whether the application of motivating elements of computer games has enabled the achievement of better results in terms of student knowledge. Also, motivation of students who used a gamified e-course was analyzed. Chapter "1. Introduction" - describes and defines the research problem and the subject of this dissertation. The research objectives and hypotheses are outlined, as well as the picture of the course of research and a description of the theoretical and empirical part of the research are included. Chapter "2. Pedagogical and Technological Aspects of E-Learning" - chapter describes the notion of e-learning and its basic elements. All the activities and resources present in the Moodle system are described. The chapter ends with a description of pedagogical aspects that have foundations in the field of e-learning. Chapter "3. Gamification of e-Learning" - within the chapters there is the theoretical descriptions and definitions of gamification as well as the basic elements that are present in gamified systems. The types of players are described and the pedagogical and psychological aspects of the flow theory on the gamified educational e-course system. Examples of computer game elements in the education system are presented, as well as a basic view of the existing gamification models. Chapter "4. E-Course Design " - chapter states the current state and appearance of the average Moodle e-Course. The author of the dissertation has developed a conceptual model of eRIOOS. According to a model, a prototype of a gamified system was made for the pilot research at the University North. With regard to the prototype, the gamified e-course was made for the first major research, at the Faculty of Organization and Informatics, on the undergraduate study in the winter semester of the academic year 2015/2015. Gamified e-course was made on the basis of conceptual solution for the second main research, conducted at the Faculty of Organization and Informatics, on the first year of graduate study in the winter semester of the academic year 2015/2015. Chapter "5. The description and features of the research " - chapter discusses theoretical considerations and outlines the aims and hypotheses of the research work. The duration of research as well as the stage of the empirical course of research is emphasized. There is also an explanation of the learning outcomes for all three surveys, together with the exam questions and final learning outcomes. The research instruments are described as well as the respondents and the content of all e-courses used in the research. The chapter ends with an explanation of the steps in preparing the research. Chapter "6. The research results " - within Chapter 6, an exhaustive review and analysis of all three researches is provided. All three studies have the same structure, the results of the pretests and posttests are analyzed. The statistical difference between the pairs of respondents is checked by computing the statistical significance of the difference by the t-test method between the average response results. The analysis of internal scale consistency is performed in all three studies and the conclusions are given for all hypothesis, sub-hypothesis and average results of students for experimental and control e-courses. Chapter "7. Discussion " - chapter highlights the preconditions and results of the research work of this dissertation. Conclusions are made for all studies and explanations are given for accepting the hypothesis H1 and H2, as well as the sub-hypothesis H1.1, H2.1, H2.2, H2.3, H2.4 and H2.5. The chapter is concluded with several similar doctoral dissertations of other researchers. Chapter "8. Conclusion " - the last chapter summarizes the methodology and the outcome of empirical research. The contributions and limitations of research are mentioned as well as an open issues and directions for future research. The dissertation ends with the conclusion, the list of literature used in theoretical part and with the end-note attachments.