Aim: To analyze the variability of palmar dermatoglyphics patterns in the Croatian population sample. Metods: The study comprised 91 participants (44 males and 47 females), i.e., 182 palmprints (91 left and 91 right hand). Each palmprints was analyzed for the presence of 25 palmar dermatoglyhic traits, 12 in the interdigital area, five in the thenar and eight in the hypothenar. The frequency of characteristics was analyzed on the individual level, side of the body and according to sex. Bilateral asymmetry and sexual dimorphism were analyzed by McNemar and χ2 test. Regression models were used to analyze the effect of the side of the body and sex on the presence and number of features. Results: All palmprints (100%) contained waterfall, clear delta, snow cone delta under ring finger, side cone delta, halfmoon, flip, and down & out. The loops between the snow cone and side delta (2.2%), loops around the clear (0.55%), around the snow cone delta under the ring finger (0%) and around the side delta (0%), vestige (11,54%), inward nose loop (10.44 %), whorl (1.1%), and arch (2.75%) appear most rarely or not at all. Most of the analyzed features did not show statistically significant bilateral asymmetry, except for the loop between the clean and snow cone deltas (P < 0.001), the loop around the snow cone delta under the middle finger (P = 0.029), vestige (P = 0.023), which appeared more often on the left side and the loop between two snow cone deltas (P = 0.001), which appeared more often on the right side. Most characteristics did not show a statistically significant difference regarding the sex of the subjects, except for the high carpal delta (P = 0.007), which was more common in females. Conclusion: Consign their high frequencies, the lack of one of the seven traits that were found on all the prints could potentially contribute to the identification/elimination of particular sample during examination. The same could also apply to extremely rare traits, especially those that were not found on any palmprint.