Fright transport in the cities has a mayor role, and it depends on successful flow of motorized and non-motorized fight vehicles with sustainable ecological and economical way. In order to achieve sustainable mobility in cities, one of the solutions imposed by the exclusion of motorized freight vehicles is the way of delivery and the introduction of bicycle logistics. Bicycle logistics is one of the ways of developing goods and goods delivery in urban centers of cities using bicycles and freight bicycles. Such a delivery system is one of the ways to achieve a new organization of traffic in cities with the aim of achieving the reduction of urban centers from motorized personal and delivery vehicles. This paper analyzes the purpose and objectives of bicycle application in delivery, and ways of delivering bicycles in urban centers. Also described are the types of delivery organization by bicycle in European cities. A survey has been conducted in the urban center of Zagreb, which will include the analysis of delivery traffic and the concept of delivery of bicycle delivery. The survey conducted in the urban center of Zagreb, which will include the analysis of delivery traffic and the concept of bicycle application in the delivery traffic. The last chapter provides an example of delivery organization by bicycle in the center of Zagreb through commercial company.