Road markings are a part of traffic signaling that serves to guide traffic, regulate traffic, warn about road conditions, warn about traffic conditions, and inform drivers about legal restrictions. One of the more important factors of the quality of pavement markings is the level of retroreflection. In order for pavement markings to maintain their quality, they must be regularly maintained, along with the visibility factors of pavement markings, i.e. retroreflection. Traffic factors, climate conditions such as precipitation, sunlight and others, and road maintenance have a negative impact on the visibility of pavement markings. There are two ways of testing retroreflection of pavement markings, namely static and dynamic retroreflection testing. The aim of this paper is to determine the impact of dynamic retroreflection testing of pavement markings on their quality. After analyzing the results of the dynamic test of retroreflection of markings on the pavement of several roads and sections of highways on different terrains in the Republic of Croatia, an evaluation of the results was carried out. After the evaluation, the influence of the dynamic retroreflection test on the maintenance of pavement markings in different parts of Croatia on roads with different volumes of traffic and environmental influences was determined. The data on the retroreflection test were obtained from the Traffic Signaling Institute, Faculty of Traffic Sciences, University of Zagreb. Finally, after proving the positive impact of dynamic testing on the quality of pavement markings, a proposal was made for optimizing the maintenance of pavement markings.