Cystotomy is a surgical procedure in which the bladder wall is opened, and is a common medical practice for the surgical treatment of the bladder. Various surgical techniques are used to close the cystotomy incision. Standard techniques imply the use of absorbable monofilament suturing materials, using simple knots or a simple continuous suture pattern with knots at the beginning and end of the suture. Due to a lack of standard suturing techniques for the cystotomy cut, such as an extended suturing time and presence of knots that can locally irritate tissues within the abdomen, there is a need for new techniques. This paper investigates the impacts of closure of the cystotomy incision, in addition to standard suturing methods, using skin staplers and barbed sutures, on the values of maximum intravesical pressure and the time needed to close the wound, while static tensile tests were used to investigate the tensile strength of the wound with regard to the closure technique. In order to obtain better insight into the ways wounds strength following different closure techniques, during the static tensile test, displacements in the wound area were measured using the Aramis optical measurement system. Additionally, the mechanical properties of porcine bladder tissue were tested in two perpendicular directions. The testing results indicated that there was a statistically significant difference in the maximum intravesical pressure between samples. Samples closed with a stapler and barbed suture achieved lower values of maximum intravesical pressure than those samples closed with standard suturing techniques. A comparison of the time needed to close the cystotomy cut showed no statistically significant differences between the standard techniques and closure technique using barbed suture, while closure using the skin stapler was significantly faster. Static tensile testing of wound strength indicated no statistically significant differences with regard to wound closure technique. Analysis of the mechanical behaviour of porcine bladder tissue in two perpendicular directions indicated that there was a statistically significant difference in the mechanical properties with regard to the sampling site. The maximum intravesical pressure measured following suturing of the wound was highest using standard techniques, and justified the use of the standard techniques used regularly in the closure of cystotomy cuts, even though the physiological intravesical pressure in porcine was lower than the values measured after the closure of the cystotomy cut using a skin stapler or barbed suture.