Knee injury is one of the most common injuries to the professional athletes, but also in recreational sport. One of the serious injuries that frequently requires surgery is the rupture of the anterior cruciate ligament. The operative procedure can be performed in several ways, one of which is taking one tendon of the hamstring of the injured person and implanting it to the site of the ruptured cruciate ligament. To obtain the required length and strength, the replacement tendon (called autograft) is folded once, twice or four times and fastened to the place of the broken ligament. This paper presents an overview of the procedures for operative treatment of ruptured anterior cruciate ligament with an emphasis on the use of the muscle tendon of the injured person. The theoretical part shows why twisting was selected as a grafting system. In the experimental part, the influence of motility on the tensile strength of the tendon was analyzed. Preliminary tests on rope and animal tendons were conducted using modern laboratory equipment, testing machines and optical measurement systems for 3D deformation analysis. It is established whether there is a difference in the tensile strength of the tendons that are folded in relation to the tendons that are folded and twisted at a defined angle.