This thesis presents the vacuum gauges calibration based on the designed measured system for comparative calibration. Described vacuum gauges can be divided according to the measurement range (rough, medium, high, ultrahigh vacuum), measurement method (direct, indirect) and gauge performance (analog, digital). For the best gauge accuracy, it is necessary to implement device calibration. Calibration implies a procedure that compares the calibrated gauges actual measurement value and the value determined by the standard. Vacuum gauge accuracy is shown by calculating the deviation and measurement uncertainty of recorded values. Measurement uncertainty values range is determined within the measured true value quantity. In the experimental part, based on the designed measuring system, the two tested calibrations pressure gauges were presented: the mechanical (with Bourdon tube) and the digital Pirani vacuum gauge. The tested pressure gauges were calibrated with reference to the standard pressure gauge - digital Pirani. The measurement results are calculated and analysed obtained and the deviations and measurement uncertainties in dependence on the standard pressure are calculated and presented graphically. By increasing the standard pressure, the deviation value also increases, and thus the measurement uncertainty value of the individual pressure gauge tested point. The analog Bourdon vacuum gauges was calibrated in the range from 0 to 130 mbar, while the digital Pirani vacuum gauges was calibrated from 10-2 to 100 mbar. The tested pressure gauges error is within 5-10%, and the inaccuracy causes are various factors such as environmental conditions, resolution, displacement, reading angle of analog pressure gauges, etc.