Human metabolism generates a heat flow that creates a thermal plume, which then affects the temperature distribution and air flow in a closed space. A variety of computer programs are used to analyze the effects, while simulation models can be validated using experimental measurements. The goal of this work is to conduct an experiment in a room at the Faculty of Mechanical Engineering and Naval Architecture in Zagreb using simplified human models, and to compare the obtained results with the results of the CFD analysis carried out in the project "Regional Competence Center Ruđer Bošković". The key parameter for the comparison of the experimental results with the simulation results was the temperature stratification above the human model. The highest temperature in the experiment was measured directly above the model (around 27°C). Also, there was a notable temperature gradient above the model. The biggest temperature difference between the measured and simulated results was closest to the model and is equal to 2°C. On other locations the temperature difference was negligible. By comparing the results of the experiment and CFD simulation, it was concluded that using simplified human models in the experiment gives results of acceptable accuracy and that the models can be used for further experimental measurements