Purpose – The purpose of the primary research is to gather information about the behaviour of bicycle tourism demand. Primarily by studying domestic bicycle tourists, the research results were expected to provide valuable market parameters, comparable with the determinants of previous relevant studies. Design and Methodology – Research was carried out by surveying different segments of bicyclists, recognized in earlier conceptual contributions. Electronic data collection techniques were applied. The online questionnaire was distributed to chosen respondents in the form of the link included in a personal message on a certain social network. A personal approach ensured a high response rate. The nature of research design resulted in a descriptive research, although some of its characteristics could be considered as exploratory. Findings – This paper is the first attempt to study the Croatian segment of the bicycle tourism demand. As far as the attributes common to both national and international research are concerned, it is possible to argue the similarities and differences of demand profiles. Value – Until there is a uniform approach to monitoring the phenomenon at the European level, the profiling of demand in bicycle tourism makes it possible to compare the specific findings with current trends. This research applies a somewhat different perspective than the existing ones – enabling more insight into the cycling-related behaviour in the home area and on vacation. As part of the international demand for bicycle tourism, knowledge about the behaviour of one particular national market contributes to the general state of the research.