Lifelong learning is a continuous long-term process in which already acquired knowledge is upgraded with new ones with the aim of improving one's own performance. The continuous progress of medicine also requires the progress of nursing, and in order to achieve this, lifelong learning and the acquisition of new professional knowledge play an important role. It is important to examine the attitudes of nursing students towards lifelong learning because they are the ones who begin their learning process in nursing and will last a lifetime. If the attitudes are positive, lifelong learning in nursing will be a logical sequence of events for that group, but if the attitudes are negative, nurses and technicians will resist the lifelong acquisition of new knowledge. The aim of this paper is to examine the attitudes of nursing students at the Faculty of Health Studies towards lifelong learning, and the initial hypothesis is that the surveyed students will have more positive than negative answers to theses on lifelong learning and professional acquisition. The research was conducted voluntarily, using an online questionnaire, and the respondents are undergraduate and graduate students of nursing in Rijeka. The research found that the respondents generally agree with most of the stated statements, which confirms the initial hypothesis. There are mostly positive responses to theses related to the importance of lifelong learning in nursing and health care in general. However, respondents have mostly negative responses to claims related to attending professional lectures and searching professional literature. It can be concluded from the research that the surveyed students are aware of the need for lifelong learning of health professionals, but for that they need better information and motivation.