Introduction: Professional burnout syndrome occurs as a result of chronic exposure to high stress in the workplace. Healthcare workers, including physiotherapists, have proven to be more susceptible to this syndrome than other professions. It has been demonstrated that physical activity has a protective effect on stress and professional burnout in certain healthcare professions. Therefore, the main aim of this research was to investigate the relationship between the level of physical activity and the intensity of professional burnout among physiotherapists. The specific aim was to compare the intensity of professional burnout among physiotherapists with regard to their level of education. Respondents and methods: The survey included 170 physiotherapists who worked in Croatia at the time of the research and had at least one year of professional experience. Besides general sociodemographic dana, the questionnaire used in this research included 2 standardized questionnaires: „Questionnaire of the intensity of burnout at work“ and „International physical activity questionnaire – short form“. Results: The results of the research demonstrated that there was no statistically significant relationship between the level of physical activity and the intensity of professional burnout among physiotherapists (p = 0,255). Furthermore, no statistically significant difference was found in the intensity of professional burnout among physiotherapists with regard to their level of education (p = 0,642). Conclusion: The research found that physical activity is not associated with professional burnout syndrome among physiotherapists in Croatia. Further research on this topic is needed in order to maintain health and work ability of physiotherapists.