Introduction: Coronavirus disease 2019 (COVID-19) is the name of the disease caused by the new coronavirus SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus-2). The virus first appeared in December 2019 in the city of Wuhan, China, and since then it is still spreading around the world. The COVID-19 pandemic has caused major and unexpected changes in the quality of physical and mental health of both healthy people and people with multiple sclerosis (MS). MS is an autoimmune chronic inflammatory demyelinating disease of the central nervous system that leads primarily to physical disability. MS sufferers are a particularly vulnerable group of people, because the lack of physical and psychosocial activities due to social isolation during the COVID-19 pandemic can have fatal consequences for their health. Aim: To determine the difference in physical and psychological limitations in performing daily activities and the impact of pain on usual work between a group of MS patients and healthy people before and after the COVID-19 pandemic. Methods and participants: The research was conducted online through an anonymous questionnaire. 23 people with MS and 16 healthy people were included in the study. Results: A statistically significant difference was found in MS patients achieving less than desired due to physical health before and after the pandemic (p = 0.049), that is, MS patients achieved less than desired due to physical health after the pandemic. MS sufferers achieved less than desired due to physical health and had greater physical limitation and greater limitation in work due to pain before and after the COVID-19 pandemic than healthy people. No statistically significant difference was found in achieving less than desired due to psychological health, nor in psychological limitation during the day before and after the pandemic in healthy and MS patients. Conclusion: The results indicate the importance of physical activity in maintaining physical fitness and reducing physical limitations in everyday life for MS patients.