This study aimed to examine the level of knowledge about hyperbaric oxygen therapy (HBOT) in wound healing among healthcare and non-healthcare professionals. A total of 140 respondents participated in the research, of which 70 were non-health workers and 70 were health workers. Differences in the level of knowledge between these two groups were analyzed, as well as the influence of the level of education on their knowledge. The results showed significant differences in the level of knowledge between health and non-health workers. Healthcare professionals were more familiar with HBOT, with 80 % having encountered this therapy during education, compared to 11.43 % of non-healthcare professionals. Also, healthcare professionals better understood the application of 100 % oxygen at pressure higher than atmospheric and the recognition of the types of chambers for HBOT. The second hypothesis, that the level of knowledge of health professionals about HBOT does not depend on the level of education, was also confirmed. The majority of healthcare professionals have secondary education, and the difference in knowledge between them and those with higher education is not significant, which indicates the importance of continuous professional development and specific training. The third hypothesis, that the level of knowledge of non-health care professionals about HBOT depends on the level of education, was also confirmed. Non-health professionals with a higher level of education had slightly better knowledge about HBOT, but the overall level of knowledge among this group was lower compared to healthcare professionals. In conclusion, the research indicated the need for better education and information, especially among non-healthcare professionals, in order to improve the understanding and application of HBOT. Also, most respondents believe that patients should be well informed about the HBOT procedure, and that working in a hyperbaric chamber is stressful and demanding. These findings highlight the importance of continuing education and support for healthcare professionals in the application of HBOT.