Various injuries to the perineal tissues can occur during vaginal birth, and most women experience some form of trauma during labour. Perineal injuries can be caused either spontaneously or intentionally and can lead to short or long-term problems for women. Various perineal protection techniques can help to reduce perineal trauma and its negative consequences. The main aim of this research was to investigate the techniques used for perineal protection in the Republic of Croatia. It is a qualitative study in which data were collected through semi-structured interviews. Ten participants took part in this research. The inclusion criteria were that the participants were employed in public health facilities in the Republic of Croatia and had worked or had worked in labour rooms for at least two years within the last five years. The collected data were analyzed using thematic analysis. In response to the first research question „Which perineal protection methods are described by midwives in the Republic of Croatia?“, the following themes were identified: manual perineal protection methods used during birth of the fetal head, perineal protection methods used during rotation and birth of the shoulders, perineal massage and use of lubricants, birth positions as perineal protection methods and complementary perineal protection methods. When describing the differences between perineal protection methods, midwives noted that the method used for perineal protection can be influenced by various factors, such as the appearance and quality of the perineum, the condition of the fetus or the use of various interventions during labour. The decision as to which method of perineal protection a midwife uses during labour is influenced by the presence of doctors and other midwives at the birth, the relationship between the woman and the midwife and the professional development of the midwife. This research is the first qualitative study on perineal protection methods in the Republic of Croatia and provides important insights into the techniques used by midwives in Croatia. Midwives should focus on continuous education and the development of evidence-based skills for perineal protection. National guidelines for perineal protection are needed in Croatia.