Human population growth and industrial development have led to an exponential need for demand and consumption of pharmaceuticals, pesticides, chemicals in production processes which results in more frequent occurrence of various pollutants in the environment. Excessive consumption has caused an increase in illegal dumping sites as well as the heavy use of pesticides and other substances, which contribute to the spread of micropollution (next: MP) in the environment. Conventional wastewater treatment methods are often not effective enough in removing MPs due to the dimensions of the compounds and their low concentrations. MPs are defined as biological or chemical contaminants present in groundwater and surface water in concentrations expressed in micrograms per liter (μg/L) and nanograms per liter (ng/L). In this research, the identification of MPs present in the Bednja river basin was carried out. Samples were collected at seven different locations in February and April 2024 in order to compare the occurrence of certain compounds. Data collected in the same period in 2023 were also taken into account. The analysis of the composition of the aquatic environment was carried out using the hybrid LC/MS Q-TOF (liquid chromatography quadrupole time-of-flight mass spectrometry) methodology. This technique uses a device that combines mass spectrometry and liquid chromatography.