The aim of the paper is to present an understanding of organizational culture and organizational structure, two key concepts in contemporary interpretation of the structure of business entities. For work purposes it was conducted research of construction company AB gradnja d.o.o. with a view to determining their structure and culture and mutual conditioning and influence on the overall business. The results show that this company has a well-structured organization where they are clear certain positions and tasks of individuals, that is, a visible and defined formal position hierarchy enabling a successful business without downtime. The functional structure of the organization is recognized made up of five departments required for seamless operation. Despite the characteristic of the structure division of labor and specialization, excellent communication between departments and multidisciplinary. Their organizational culture is classified as a combination of all four types according to the classification of authors Quinn and Cameron. It is characterized by a strong commitment to theirs employees and developing human resources, which makes them attractive in the job market, especially for highly educated candidates and results in a large number of open job applications received. Throughout the year, the company has open tenders, which enables them to succeed business, a large number of new and upcoming projects, constant advances in construction technology and organization performance. It is the achievements of individuals, viewed individually or as a whole team, with a wide range of opportunities for self-realization and the realization of the maximum, a factor that overall elevates the organization to a higher level.