Paper is the most common printing medium for printing packaging and labels. As the main source of raw material for the production of paper printing substrates in the world is still wood, global awareness of the risks of forest exploitation is on the rise as is the importance of recycling and finding alternative sources of cellulose fibers. In this work, straw will be used as a by-product of cereal production and thus a potential lignocellulosic raw material for the production of printing substrate. Since aging is an inevitable process of any printing substrate but also of the printed substrate and the degradation of print quality due to aging largely depends on the properties of the printing substrate and inks. This research is focused on the analysis of the quality and optical stability of prints on printing substrate containing straw fibers after exposure to natural and accelerated aging. Press substrates for the purposes of this study were made in laboratory conditions by mixing recycled wood pulp with different proportions of straw fiber. The printing media were then printed by digital technique (UV inkjet), and after spectrophotometric measurements separated into two groups (for natural and accelerated aging in the SunTEST XLS + device). The aim of this paper is to compare and examinethe quality and optical stability of the print based on changes in colorimetric values and to defining the Euclidean color difference (ΔE₀₀) after exposure to aging.