Surface phenomena in print are extremely important for understanding and optimizing the interaction of materials involved in the graphic reproduction process. In the screen printing technique, the surface properties of the print are, among other things, related to the selection of the mesh on the printing form, since the rough surface of the print due to the mesh structure itself can significantly affect the free surface energy of the printed ink layer. In order to protect porous printing substrates from moisture penetration, to strengthen mechanical properties or to ensure better adhesion of the printing ink to the substrate, the substrates are often coated with protective coatings before printing. Then the adhesion parameters between the coating and the printing ink become extremely important for assessing the durability, but also the quality of the print. In this work, biodegradable coatings (polycaprolactone and polylactic acid) were used, which were applied to a paper substrate in order to reduce the permeability to water vapor. Control motifs were then printed on the substrate using two types of water-based screen printing inks: ink with transparent white, and ink with cover white. Before printing, the optimal line of the screen printing grid for the selected motifs was experimentally determined. By analyzing the surface and interfacial properties in the system "printing substrate-coatingprint" and measuring the properties of the print surface such as roughness and visual analysis by 2D and 3D microscopy, it will be possible to describe and optimize the interaction of biodegradable coating and printing inks for screen printing.