This study examined the construction of the discourse on gender ideology in Croatia and the impact that the Statement of the Scientific Council for Education and School System of the Croatian Academy of Sciences and Arts had within the discourse and on the broader social practices. The discourse on gender ideology has been constructed within the populist framework in terms of invocation of the general will of the people, constructing conflicts between political elites and people, and rejecting expert knowledge. The discourse has an ideological and value basis, expressed through gender ideology and re-contextualized within the discourses of various social fields, institutions, and organizations. This recontextualization indicates the shifting of discursive boundaries between different social fields – politics, media, civil society, the academic community, and the Catholic Church – on the common ground of rejecting the dominant liberal discourse. The Statement introduces a new momentum in the discourse on gender ideology, at the same time re-contextualizing the existing populist and conservative discourse and moving the discursive boundaries further into the fields of science and education. Social and political practices such as the political decision-making within international and supranational organizations or communities, the disappointment in liberal values or inability to associate with them, have proved constitutive for the discourse on gender ideology, and at the same time, the discourse on gender ideology has been constitutive for broader social practices in the sense of further strengthening the conservative coalition, in the mobilization effect for protest or referendum initiatives, but also for the formal legal framework of the State, as gender ideology is recognized and integrated into the legislation.