The aim of this study was to examine to what extent do students of helping professions support the rights of lesbian and gay men and to assess determinates for support of these rights. The sample consisted of 676 students who completed the questionnaire during their regular classes. The questionnaire consisted of questions about socio-demographic characteristics, Support for Gay and Lesbian Civil Rights scale, Modern Homonegativity scale, Short version of the Right-Wing Authoritarianism scale, Social Dominance Orientation scale, Short religiosity scale and the question about the number of contacts with lesbians and gay men. The results showed that students moderately support the rights of gay men and lesbians. Female students are significantly more supportive of gay and lesbian rights than male students, while there is no difference in supporting lesbian rights in comparison to the rights of gay men. Support for gay man and lesbian rights is greater if people have less homonegative attitudes, if they are less oriented to group-based dominance, opposition to equality and right-wing authoritarianism, if they are less religious, left on political orientation and have more contact with lesbians and gay men. The results of hierarchical regression analysis have shown that support for lesbian rights is best predicted by modern homonegativity, right-wing authoritarianism, opposition to equality and group-based domination, and support for the rights of gay men by modern homonegativity, right-wing authoritarianism, political orientation, number of contacts, and significantly but weakly by group-based domination and opposition to equality. Since support for the rights of these populations has been poorly examined in Croatia, additional research is needed to assess the determinants of supporting the rights of gay men and lesbians and, consequently, to reduce discrimination.