With the outbreak of the COVID-19 pandemic a year ago, people’s lives changed in many aspects. The virus has affected various aspects of an individual's life in the form of the appearance of various forms of restrictions that have affected an individual's quality of life. One aspect of life in which there were/are also many changes is the work context. Given that work is necessary for existence and covers a large part of the day or life of a person, it is important to study in more detail the changes that have occurred in this field which can be related to the quality of life of employees. Because of this reason that this research was conducted on the topic of employee quality of life focusing on changes in perceived levels of stress and subjective well-being with respect to changes in the way we work during a pandemic. A total of 156 employees from different parts of the Republic of Croatia participated in the research. The survey was conducted in the period from May to July 2021 in online form. The questionnaire covered sociodemographic data, conditions in which they (employees) performed work from home (form of teaching children, work of other family members, etc.), Questionnaire of life satisfaction, Scale of perceived stress, Questionnaire of new ways of working and Scale of family – work conflict. The results indicated a moderate perceived level of stress and a moderate level of subjective well-being. In general, both men and women assessed a greater disruption of the work role to the family than vice versa. Also, perceived stress and subjective well-being were shown to be statistically significantly associated with work-family conflict and familywork conflict.