The aim of this research was to investigate differences in empathy among participants based on their field of study and gender. The main goal was to examine the impact of music on empathy in different conditions. The study involved 450 students from the Zagreb County, aged 18 to 58 years (176 male, 271 female, 3 subjects did not disclose their sex). Participants were divided into groups using probabilistic methods to balance variability factors. Data were collected via a Google form under researcher supervision. Questionnaires used in this study are the E-questionnaire (Raboteg-Šarić, 2002) that measures two components of empathy and a custom measure of empathic response. Due to significant distribution abnormalities, non-parametric tests were mostly used. Students in helping professions scored higher on empathy measures compared to those in non-helping professions, even when controlling for sex. Female participants scored higher than male participants on empathy measures. The impact of music on empathy was found in the first condition; melancholic music facilitated empathy in sad content recordings but with a somewhat smaller effect size. No effect of music was found for second condition. The group that listened to a happy story with melancholic music differed from the control group before the experimental intervention, so it was not justified to comment on the differences after the introduction of the independent variable. The experimental group that listened to a happy story in the same condition did not significantly differ from the control group after the intervention. Further research is recommended to investigate individual differences in empathy but using various measurement methods. In understanding the relationship between music and empathy, there remains room for enhancing current findings.