Vitamin E is an organic substance soluble in fats. It is very important for human health and since the human body cannot produce it on its own, it is necessary to intake it through food or dietary supplements. It is also an important food supplement in laying hens feed due to the maintenance of physiological functions and egg production and in this way the intake of vitamin E in the human body increases. The aim of the study was to determine the content of vitamin E in laying hens feed and hens feed enriched with vitamin E and various oils. Three different methods were used: direct extraction with methanol, direct ultrasound extraction and extraction with prior saponification. After extraction, samples were analyzed by means of high-performance liquid chromatography. Concentration of vitamin E was determined in samples of hens feed available on the market, samples with added oils, with the addition of other nutricines (selenium and lutein) and food with the addition of vitamin E and other nutricines. The highest concentration of vitamin E was found in samples with the addition of vitamin E and other nutricines (725.940 mg/kg of hens feed) and the lowest concentration of vitamin E was found in samples with the addition of other nutricines (selenium) 193.242 mg/kg of hens feed.