The aim of this thesis was to determine the differences in the level of performance during change of direction with and without the ball among female football players and to establish the connection between variable for estimated direction change speed and variable for estimated explosive power like sprint with and without ball. The measurement was carried out on a sample of 33 players, aged 18-27 (average 21.44 ± 3.18a ages), height 160-183 centimeters (168.69 ±5.03 cm), and weight 53-79 kg (61.88 ± 6.18 kg). The following descriptive indicators were measured: arithmetic mean (AM), standard deviation (SD), the minimum (MIN) and the maximum (MAX) result. Variables that were analyzed determine: 20 meter sprint (3.63±0.23s without ball, 4.18±0.31s with ball), 93639 forward-backwards (8.83±0.52s without ball, 11.33±0.73s with ball), 93639 with a turn (8.14±0.39s without ball, 10.38±0.67s with ball), slalom (7.49±0.89s without ball, 11.16±1.20 with ball), zig-zag (6.09±0.49s without ball, 8.94±0.76s with ball) and 20 yard sprint (5.23±0.25s without ball, 6.31±0.33s with ball). The utilized method was the dependent samples t-test which analyzed differences significance between change of direction without the ball compared to change of direction with the ball. According to the results, we can conclude that there is a significant difference between the tests with and without the ball and that the ball dribbling increases tested performance time. Female football players without ball dribbling are faster and achieve higher speed at direction change compared to players with ball dribbling. The smallest difference was noticed at the MES20M test, which was expected considering test simplicity, regarding the fact that the test is performed without direction change. The differences increase as the complexity of test performance increases so the biggest difference obtained was in the slalom test (MAGSL). This test requires the largest amount of changes of direction at an angle, resulting in a loss of ball dribbling speed. The players completed this test 3.67 s, i.e. 32.9 %, faster without dribbling the ball. The 20 yard sprint yielded the smallest difference in change of direction. This test includes only two changes of direction and the players completed it 1.08 s, or 18.7 %, faster when not dribbling the ball. The Pearson correlation coefficient was used to measure the correlation of variables. Similar tests have a higher correlation degree so for example tests with direction change and 180 degree turns are in higher correlation, both with and without ball.