Introduction: Some researches point to possibility of improving a cyclist's abilities with a specific breathing training, but other works have not confirmed value of a breathing training and there is no common attitude towards that. Aim: The aim of the research is to test influence of the specific respiratory muscles training over the period of 8 weeks, conducted at the same time with standard cycling training, on dynamic lung capacities, cycling economy and parameters for evaluation of aerobic capacity and competitive success. Methods: The sample of respondents consists of 30 cyclists of the national rank sorted randomly into experimental and control group. Both groups have conducted a standard cycling training over the period of eight weeks and the experimental group has additionally conducted a specific respiratory muscles training: 1) training for voluntary control of breathing, 2) training for strengthening the respiratory muscles. Before and after the experimental procedure all respondents have conducted a measurement of dynamical lung capacity and three tests, two laboratory tests on bicycle ergometer: 1) a test with a constant burden of 150 W during the period of 10 minutes in order to estimate cycling economy (C150), 2) a progressive test of the burden in order to estimate the aerobic capacity (GXT VO2max) and one field test: 3) a maximal test of a bicycle ride on a 4 km long cycling track in order to estimate competitive success (t4km). Statistically significant difference between the initial and final measurement in the experimental and control group have been tested with the t-test for dependent samples and the difference between grups ANOVA for repeated measures (p < 0,05). Results: In comparison to the initial measurement (I), after the conducted training programme and the respiratory muscles training the following has been determined among the respondents from the experimental group in the final measurement (F): 1) significantly shorter cycling time in the field test (t4km_I = 393,4 ± 19,1 : t4km_F = 386,1 ± 12,9 (s) p < 0,05); 2 ) significantly higher willing ventilation (MMV_I = 184,1 ± 31 : MMV_F = 199,8 ± 33,3 (l) p < 0,05); 3) better cycling economy with the burden of 150 W (C150_I = 0,198 ± 0,044 : C150_F = 0,182 ± 0,038 (ml/kg/min/W) p < 0,05); 4) significantly higher values of the parameters for evaluation of aerobic capacity at the anaerobic threshold (ANP_I = 304,7 ± 43,1 : ANP_F = 326,0 ± 32,5 (W) p < 0,05) and maximum intensity (Wmax_I = 353,3 ± 44 : Wmax_F = 378,6 ± 31,5 (W) (p < 0,05). At the control group of the respondents no statistically significant differences for the mentioned parameters have been determined. Conclusion: The results of this research point to the fact that a specific breathing training directed to improvement of breathing technique and strengthening of respiratory muscles (over the period of 8 weeks together with a standard cycling training) time improvement on the 4 km long cycling track, leads to increase of maximum willing volume, increase in cycling economy at 150 W, increase in the value anaerobic threshold and and maximum intensity.