The main goal of this research was to determine the effects of the fine motor skills program in relation to the gross motor skills program (multilateral program) on physical activity, motor skills and abilities of preschool children. The partial goal of this research was to determine the effects of the exercise program on overall child development. The study involved 151 boys and girl aged 5 to 7, and their parents and educators from two randomly selected kindergartens in the city of Zagreb. The sample included only children, parents and educators who completed the initial and final measurements (N=120). Each kindergarten chose three groups that participated in the research. A multilateral exercise program was implemented in experimental group 1 (N=43), a fine motor program in experimental group 2 (N=44) and the third group was a control group (N=33). At the very beginning of the research, parents completed the "Netherlands Physical Activity Questionnaire" (NPAQ), a questionnaire about their child's physical activity and sedentary activities. Also, parents and educators of kindergarten groups that children participated in the research completed the "Parent's Evaluations of Children's Developmental Status" (PEDS), related to the assessment of children's developmental status. The motor skills and abilities of preschool children included in the research were tested by two sets of tests "Test of Gross Motor Development - Second Edition" and "Bruininks-Oseretsky Test of Motor Proficiency - Second Edition "(BOT-2). The measurement with all the mentioned questionnaires and tests was conducted on at the beginning and at the end of the experimental procedure. The results showed that the children in experimental group 1, after the intervention of the multilateral exercises program, achieved significantly better results in tests for assessing motor skills (gross motor quotient of the TGMD-2 test and standardized locomotor skills of the TGMD-2 test). In the mentioned group, there were no significant effects in the motor index of the BOT-2 test, but only a numerical improvement. After the fine motor intervention in experimental group 2, no significantly better results were achieved in the tests for assessing fine motor skills (fine motor integration and fine motor precision of the BOT-2 test). The control group makes progress in the motor index of the BOT-2 tests between the initial and final measurements. The level of physical activity did not change significantly in any group between the two measurement points. Namely, a significant decrease in sedentary activities was registered only in the fine motor skills group after the final measurement. Furthermore, no group recorded significant changes in the children's developmental status as assessed by educators. The assessment of the parents recorded significant changes in the developmental status of the children, more specifically, a decrease in the level of concern was determined after the interventions in the group of the multilateral exercises program and the fine motor program. This research enabled the evaluation the effect of two different programs (a multilateral exercises program and a fine motor program) on children's motor skills and abilities, as well as on the level of physical activity, sedentary activities, and the overall development of preschool children.