Lack of physical activity could lead to many health issues regardles of age and gender. The purpose of this study was to determine functional movement in urban adolescents and to examine gender differences. The study was conducted on a large, representative sample (n=730) of adolescent population (16-17 yrs). We used the functional movement screen (FMS™) for the assessment of the functional movement. It is screening tool which evaluates seven basic movements that are fundamental for the functional movement. Results show slightly better scores among girls compared to boys (12,7 vs. 12,2; p=0,006). In addition, differences were noted in several individual functional movement patterns, but not regarding the number of assymetries. Specifically, boys showed better results in pushup and rotary stability tests, whereas girls outperformed boys in inline lunge, active straight leg raise and shoulder mobility tests. The remaining two movements, deep squat and hurdle step faild to show gender differences in score distribution. All in all, these results show that movement quality deficit is highly prevalent among adolescents. The observed movement patterns, especially the lack of mobility and stability could lead to increased risk of injuries related to physical activity. Thus, the quality precedes the quantity in terms of physical activity and functional movement is synonym for biomechanicaly correct movement and in such a manner should be implemented more frequently. The results of present study should be considered while using FMS as a movement quality assessment among adolescent population.