The purpose of this research was to examine the relationship between physical activity, physical and global self-concept of students, as well as gender differences. 131 undergraduate and graduate students of the Faculty of Kinesiology Osijek participated in the research. The International Physical Activity Questionnaire - short version (Craig et al., 2003) and the Physical Self-Description Questionnaire (Marsh et al., 2010) were used. Based on the obtained results, it was shown that the majority of students participate in vigorous-intensity physical activities (89.31%). It was found that male students in general spend significantly more time in physical activities than female students. Male students also engage in vigorous-intensity physical activities significantly more than female students. Regarding self-concept, male students rate their general physical self-concept significantly higher than female students. No significant gender differences were found in self-esteem, nor was self-esteem related to physical activity. However, a significant positive association was obtained between a vigorous-level of physical activity and physical self-concept, as well as between total physical activity and global physical self-concept. Students who in general spend more time in physical activities as well as vigorous-intensity activities report higher physical self-concept.