Systematic monitoring of sea water quality represents exceptional public health importance for the Republic of Croatia, whose economic development is based on tourism. In this paper, the microbiological load of Luke Rijeka, as the largest Croatian port of the northern Adriatic, was examined. In the period from 11.09.2014. to 11.11.2015. ,seven sea water and sediment sampling were carried out at port of Rijeka on 4 locations: LRI01, LRI04, LRI05 and JPG28. Except for the indicators prescribed by the Ordinance on ballast water management and control (NN 128/2012), E. coli, Enterococci and V. cholerae (O1 and O139), there were also investigated additional microbiological indicators: C. perfringens, P. aeruginosai S. aureus, Salmonella spp., Shigella spp. Enteroviruses. The test results showed that there are no significant seasonal variations (with the most loaded winter period). The highest microbiological load was found at locations LRI01 and LRI04. The water concentration of E. coli is 2.5 times, and the enterococci is 1.5 times higher than the sediment (log cfu 100 / g). In the area of port of Rijeka concentrations of indicators of fecal contamination are almost two orders of magnitude larger than the surrounding area. Of the additional microbiological indicators, the highest number was found for C. perfringens, whose concentrations in the sediment are higher than in the water. P. aeruginosa appears in a smaller number (higher concentrations in water than in sediment), and S. aureus is present in the smallest number (only in water, while in the sediment it has not been proved). Salmonella spp was detected in 29% of the tested water samples (8/28). Sgihella spp and enteroviruses were not detected in any sample. The results show that there is a constant source of fecal pollution in the area of port of Rijeka, which is why seasonal variations in the number of fecal bacteria do not occur, and therefore a large number of sea water samples do not comply to the prescribed criteria. The results show changeable but significant fecal contamination of sea water and sediment in the area of port of Rijeka.