The use of large pesticide quantities, as well as their high water solubility, represent a significant environmental hazard, in the form of water, soil, air pollution and disruption of the ecosystem homeostasis. For that reason, the aim of this study was to investigate the effect of soil characteristics from following locations: Grobnik, Matulji, Međimurje and Otočac with the main emphasis on the effect of organic carbon soil characteristics on the sorption behavior of dimethoate. Actual, exchangeable and hydrolytic acidity of soil, character and humus content, amount of carbon in extract, humic and fulvic acids and spectrophotometric determination of the natural humic acid structure at wavelengths of 465 and 665 nm were performed. Determination of the soil sorption capacity for dimethoate was performed using a high performance liquid chromatograph (HPLC). The soil reactions were neutral and alkaline (pH=6.73-7.40) and the proportion of organic matter content in soil ranged from 1.21-3.47% classifying soils as poorly and fairly humus soils. According to Freundlich's model for describing the sorption process of organic compounds in soil, sorption isotherms were classified as C type for the samples GR, M2 and L1, L type for the sample MEĐ and S type for the sample M1. Furthermore, the values of the KF coefficient varied from 0.20-6.91 (mg/kg) (mg/L)1/n. The results of the study indicate that the sorption behavior of dimethoate in soil is not only caused by soil organic matter content, than by the combined effect of all soil characteristics.