prikaz prve stranice dokumenta A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae
  Preuzmi
PDF 1.81 MB
Znanstveni rad - Izvorni znanstveni rad
A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae
Scientific Reports, 8 (2018); 6340. https://doi.org/10.1038/s41598-018-24724-1
Best, Ashley; Price, Christopher; Ožanič, Mateja; Šantić, Marina; Jones, Snake; Abu Kwaik, Yousef

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

Citirajte ovaj rad

Best, A., Price, C., Ožanič, M., Šantić, M., Jones, S. i Abu Kwaik, Y. (2018). A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae. Scientific Reports, 8.. doi: 10.1038/s41598-018-24724-1

Best, Ashley, et al. "A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae." Scientific Reports, vol. 8, 2018. https://doi.org/10.1038/s41598-018-24724-1

Best, Ashley, Christopher Price, Mateja Ožanič, Marina Šantić, Snake Jones i Yousef Abu Kwaik. "A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae." Scientific Reports 8 (2018). https://doi.org/10.1038/s41598-018-24724-1

Best, A., et al. (2018) 'A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae', Scientific Reports, 8. doi: 10.1038/s41598-018-24724-1

Best A, Price C, Ožanič M, Šantić M, Jones S, Abu Kwaik Y. A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae. Scientific Reports [Internet]. 20.04.2018. [pristupljeno 11.05.2024.];8. doi: 10.1038/s41598-018-24724-1

A. Best, C. Price, M. Ožanič, M. Šantić, S. Jones i Y. Abu Kwaik, "A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae", Scientific Reports, vol. 8, Travanj 2018. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:122051. [Citirano: 11.05.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:122051
Podaci o radu
Naslov (engleski)A Legionella pneumophila amylase is essential for intracellular replication in human macrophages and amoebae
AutorAshley Best
AutorChristopher Price
AutorMateja Ožanič
AutorMarina Šantić
AutorSnake Jones
AutorYousef Abu Kwaik
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za mikrobiologiju i parazitologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Medicinska mikrobiologija
Sažetak (engleski)
Legionella pneumophila invades protozoa with an “accidental” ability to cause pneumonia upon transmission to humans. To support its nutrition during intracellular residence, L. pneumophila relies on host amino acids as the main source of carbon and energy to feed the TCA cycle. Despite the apparent lack of a requirement for glucose for L. pneumophila growth in vitro and intracellularly, the organism contains multiple amylases, which hydrolyze polysaccharides into glucose monomers. Here we describe one predicted putative amylase, LamB, which is uniquely present only in L. pneumophila and L. steigerwaltii among the ~60 species of Legionella. Our data show that LamB has a strong amylase activity, which is abolished upon substitutions of amino acids that are conserved in the catalytic pocket of amylases. Loss of LamB or expression of catalytically-inactive variants of LamB results in a severe growth defect of L. pneumophila in Acanthamoeba polyphaga and human monocytesderived macrophages. Importantly, the lamB null mutant is severely attenuated in intra-pulmonary proliferation in the mouse model and is defective in dissemination to the liver and spleen. Our data show an essential role for LamB in intracellular replication of L. pneumophila in amoeba and human macrophages and in virulence in vivo.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaScientific Reports
Brojčani podacivol. 8, 6340
e-ISSN2045-2322
DOIhttps://doi.org/10.1038/s41598-018-24724-1
URN:NBNurn:nbn:hr:184:122051
Datum objave publikacije2018-04-20
ProjektŠifra: IP-2016-06-9003
Naziv (hrvatski): Uloga unutarstaničnog života Francisella tularensis u patogenezi eksperimentalne tularemije
Kratica: FRANCYCLE
Voditelj: Marina Šantić
Pravna nadležnost: Hrvatska
Financijer: HRZZ
Linija financiranja: IP
URL dokumentahttp://www.nature.com/articles/s41598-018-24724-1
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2020-02-02 17:50:50