According to the literature, quality of life has been shown to be reduced in females compared with males with Inflamatory Bowel Disease (IBD) in most studies. Furthermore, psychosocial factors are also playing an important role in IBD, especially emotional lability. The aim of this study was to investigate the sex differences in general and specific health-related quality of life (HRQoL), anxiety and depression in IBD patients. Hundred and twelve outpatients of the Gastroenterology Department, Clinical Hospital Centre Rijeka, were enrolled in this study. There were 50 females (31 with ulcerative colitis, UC and 19 with Crohn disease, CD) and 62 males (30 with UC and 32 with CD), age range 19 to 74 (M=41.46 ; SD=13.06). Most patients have been in long clinical remission or with mild disease acording to Clinical Disease Activity Index (CDAI) score for CD and Clinical Activity Index ( CAI) score for UC. There were significant differences in physical (F=13.96, p<.0001) and mental (F=9.44, p<.001) component of the general HRQoL, emotional domain ((F=9.26, p<.001) and bowel symptoms (F=7.04, p<.001) of the Inflamatory Bowel Disease Quality of life (IBDQoL), as well as, in anxiety (F=7.03, p<.001) and depression (F=12.09, p<.0001) between men and women with IBD. Women have expressed significantly lower level of the general HRQOL and more emotional disturbances connected with their disease as well as more frequent bowel symptoms compared with men. Effect sizes of those differences were large. The results of this study confirm that women with IBD are more prone to the negative impact of the disease on their health-related quality of life than men. Women with higher level of depression and anxiety experienced more emotional disturbances, bowel and systemic symptoms and lower general health-related quality of life. These results should deserve more consideration in the clinical treatment of IBD patients.