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Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids
Biomedicines, 9 (2021), 6; 603. https://doi.org/10.3390/biomedicines9060603
Malenica, Mladenka; Vukomanović, Marija; Kurtjak, Mario; Masciotti, Valentina; dal Zilio, Simone; Greco, Silvio; Lazzarino, Marco; Krušić, Vedrana; Perčić, Marko; Jelovica Badovinac, Ivana; Wechtersbach, Karmen; Vidović, Ivona; Baričević, Vanja; Valić, Srećko; Lučin, Pero; Kojc, Nika; Grabušić, Kristina Više autora...

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Malenica, M., Vukomanović, M., Kurtjak, M., Masciotti, V., dal Zilio, S., Greco, S. ... Grabušić, K. (2021). Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids. Biomedicines, 9. (6). doi: 10.3390/biomedicines9060603

Malenica, Mladenka, et al. "Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids." Biomedicines, vol. 9, br. 6, 2021. https://doi.org/10.3390/biomedicines9060603

Malenica, Mladenka, Marija Vukomanović, Mario Kurtjak, Valentina Masciotti, Simone dal Zilio, Silvio Greco, Marco Lazzarino, et al. "Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids." Biomedicines 9, br. 6 (2021). https://doi.org/10.3390/biomedicines9060603

Malenica, M., et al. (2021) 'Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids', Biomedicines, 9(6). doi: 10.3390/biomedicines9060603

Malenica M, Vukomanović M, Kurtjak M, Masciotti V, dal Zilio S, Greco S, i sur.. Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids. Biomedicines [Internet]. 26.05.2021. [pristupljeno 24.12.2024.];9(6). doi: 10.3390/biomedicines9060603

M. Malenica, et al., "Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids", Biomedicines, vol. 9, br. 6, Svibanj 2021. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:323752. [Citirano: 24.12.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:323752
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Naslov (engleski)Perspectives of Microscopy Methods for Morphology Characterisation of Extracellular Vesicles from Human Biofluids
AutorMladenka Malenica
AutorMarija Vukomanović
AutorMario Kurtjak
AutorValentina Masciotti
AutorSimone dal Zilio
AutorSilvio Greco
AutorMarco Lazzarino
AutorVedrana Krušić
AutorMarko Perčić
AutorIvana Jelovica Badovinac
AutorKarmen Wechtersbach
AutorIvona Vidović
AutorVanja Baričević
AutorSrećko Valić
AutorPero Lučin
AutorNika Kojc
AutorKristina Grabušić
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za medicinsku kemiju, biokemiju i kliničku kemiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Extracellular vesicles (EVs) are nanometric membranous structures secreted from almost every cell and present in biofluids. Because EV composition reflects the state of its parental tissue, EVs possess an enormous diagnostic/prognostic potential to reveal pathophysiological conditions. However, a prerequisite for such usage of EVs is their detailed characterisation, including visualisation which is mainly achieved by atomic force microscopy (AFM) and electron microscopy (EM). Here we summarise the EV preparation protocols for AFM and EM bringing out the main challenges in the imaging of EVs, both in their natural environment as biofluid constituents and in a saline solution after EV isolation. In addition, we discuss approaches for EV imaging and identify the potential benefits and disadvantages when different AFM and EM methods are applied, including numerous factors that influence the morphological characterisation, standardisation, or formation of artefacts. We also demonstrate the effects of some of these factors by using cerebrospinal fluid as an example of human biofluid with a simpler composition. Here presented comparison of approaches to EV imaging should help to estimate the current state in morphology research of EVs from human biofluids and to identify the most efficient pathways towards the standardisation of sample preparation and microscopy modes.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Pregledni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBiomedicines
Brojčani podacivol. 9, br. 6, 603
e-ISSN2227-9059
DOIhttps://doi.org/10.3390/biomedicines9060603
URN:NBNurn:nbn:hr:184:323752
Datum objave publikacije2021-05-26
URL dokumentahttps://www.mdpi.com/2227-9059/9/6/603
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje 4.0 međunarodna (CC BY 4.0)
Datum i vrijeme pohrane2021-12-03 11:33:26