prikaz prve stranice dokumenta Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet
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Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet
Antimicrobial Agents and Chemotherapy, 41 (1997), 12; 2686-2692. urn:nbn:hr:184:610927
Pavić, Ivica; Hartmann, Anke; Zimmerman, Albert; Michel, Detlef; Hampl, Walter; Schleyer, Ingrid; Mertens, Thomas

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

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Pavić, I., Hartmann, A., Zimmerman, A., Michel, D., Hampl, W., Schleyer, I. i Mertens, T. (1997). Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet. Antimicrobial Agents and Chemotherapy, 41. (12), 2686-2692. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:184:610927

Pavić, Ivica, et al. "Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet." Antimicrobial Agents and Chemotherapy, vol. 41, br. 12, 1997, str. 2686-2692. https://urn.nsk.hr/urn:nbn:hr:184:610927

Pavić, Ivica, Anke Hartmann, Albert Zimmerman, Detlef Michel, Walter Hampl, Ingrid Schleyer i Thomas Mertens. "Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet." Antimicrobial Agents and Chemotherapy 41, br. 12 (1997): 2686-2692. https://urn.nsk.hr/urn:nbn:hr:184:610927

Pavić, I., et al. (1997) 'Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet', Antimicrobial Agents and Chemotherapy, 41(12), str. 2686-2692. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:184:610927 (Datum pristupa: 18.05.2024.)

Pavić I, Hartmann A, Zimmerman A, Michel D, Hampl W, Schleyer I, i sur.. Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet. Antimicrobial Agents and Chemotherapy [Internet]. 1997. [pristupljeno 18.05.2024.];41(12):2686-2692. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:610927

I. Pavić, et al., "Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet", Antimicrobial Agents and Chemotherapy, vol. 41, br. 12, str. 2686-2692, 1997. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:610927. [Citirano: 18.05.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:610927
Podaci o radu
Naslov (engleski)Flow cytometric analysis of herpes simplex virus type 1 susceptibility to acyclovir, ganciclovir, and foscarnet
AutorIvica Pavić
AutorAnke Hartmann
AutorAlbert Zimmerman
AutorDetlef Michel
AutorWalter Hampl
AutorIngrid Schleyer
AutorThomas Mertens
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za zarazne bolesti)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Infektologija
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
We established a quantitative flow cytometric method for determination of herpes simplex virus type 1 (HSV-1) susceptibility to acyclovir (ACV), ganciclovir, and foscarnet in vitro. Susceptibility was defined in terms of the drug concentration which reduced the number of cells expressing HSV-1 glycoprotein C (gpC) with a fluorescence intensity of > or =10(2) by 50% (IC50). Flow cytometry allowed us to use a high (1.0) as well as a low (0.005) multiplicity of infection, and determination of the IC50 was possible after one or more viral replicative cycles. IC50s were dependent on virus input and on time postinfection. In mixture experiments, 1 to 2% resistant viruses added to a sensitive strain could be detected. The results obtained by flow cytometry showed a good qualitative correlation with those achieved by cytopathic effect inhibitory assay. However, flow cytometry might detect more quantitative differences in drug susceptibility, especially among resistant strains, as confirmed also by determination of intracellular drug phosphorylation. The mean IC50s for ACV-sensitive strains were 0.45 to 1.47 microM, and those for ACV-resistant strains were between 140 and 3, 134 microM. Flow cytometric analysis was fast and accurate, automatizable, and highly reproducible. Flow cytometry may be a more powerful tool than standard cytopathic effect-based assays and could have advantages for the detection of low levels of drug resistance or mixtures of sensitive and resistant virus strains.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran - međunarodna recenzija
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaAntimicrobial Agents and Chemotherapy
Brojčani podacivol. 41, br. 12, str. 2686-2692
p-ISSN0066-4804
URN:NBNurn:nbn:hr:184:610927
Datum objave publikacije1997
URL dokumentahttp://bib.irb.hr/811830
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Zaštićeno autorskim pravom.
Datum i vrijeme pohrane2022-04-20 14:01:53