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Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions
Na: 46th International Herpesvirus Workshop. Virtual meeting, Sjedinjene Američke Države, 2022. urn:nbn:hr:184:244380
Marcelić, Marina; Mahmutefendić Lučin, Hana; Jurak Begonja, Antonija; Blagojević Zagorac, Gordana; Viduka, Ivona; Lučin, Pero

Institucijski repozitorij: Repozitorij Medicinskog fakulteta u Rijeci

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Marcelić, M., Mahmutefendić Lučin, H., Jurak Begonja, A., Blagojević Zagorac, G., Viduka, I. i Lučin, P. Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions [PowerPoint prezentacija]. Preuzeto s https://urn.nsk.hr/urn:nbn:hr:184:244380

Marcelić, Marina, et al. "Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions." 46th International Herpesvirus Workshop, 25.07.2022.-29.07.2022., Virtual meeting, Sjedinjene Američke Države. Prezentacija. https://urn.nsk.hr/urn:nbn:hr:184:244380

Marcelić, Marina, Hana Mahmutefendić Lučin, Antonija Jurak Begonja, Gordana Blagojević Zagorac, Ivona Viduka i Pero Lučin. "Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions." Prezentacija sa skupa 46th International Herpesvirus Workshop, Virtual meeting, Sjedinjene Američke Države, 25.07.2022.-29.07.2022. https://urn.nsk.hr/urn:nbn:hr:184:244380

Marcelić, M., et al. 'Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions' [Prezentacija], 46th International Herpesvirus Workshop. 25.07.2022.-29.07.2022. Preuzeto s: https://urn.nsk.hr/urn:nbn:hr:184:244380

Marcelić M, Mahmutefendić Lučin H, Jurak Begonja A, Blagojević Zagorac G, Viduka I, Lučin P. Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions. [Prezentacija] [pristupljeno 07.11.2024.] Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:244380

M. Marcelić, H. Mahmutefendić Lučin, A. Jurak Begonja, G. Blagojević Zagorac, I. Viduka i P. Lučin, "Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions", prezentirano na: 46th International Herpesvirus Workshop, Virtual meeting, 25.07.2022.-29.07.2022. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:184:244380

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:184:244380
Podaci o radu
Naslov (engleski)Endosomal Phosphatidylinositol-3-phosphate is Essential for the Final Assembly of CMV Virions
AutorMarina Marcelić
AutorHana Mahmutefendić Lučin
AutorAntonija Jurak Begonja
AutorGordana Blagojević Zagorac
AutorIvona Viduka
AutorPero Lučin
Autorova ustanovaSveučilište u Rijeci
Medicinski fakultet
(Katedra za fiziologiju, imunologiju i patofiziologiju)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Temeljne medicinske znanosti
Sažetak (engleski)
Cytomegaloviruses (CMVs) initiate the stepwise establishment of their cytoplasmic virion assembly compartment (cVAC) at the earliest stages of infection by expanding the interface between early endosomes (EEs) and endosomal recycling compartment (ERC) and relocating the Golgi complex. This membranous organelle reorganization involves phosphatidylinositol-3-phosphate (PI(3)P)-containing membrane domains, as PI(3)P is the master regulator of the EE pathway. Our goal was to investigate the contribution of PI(3)P-dependent EE domains to cVAC biogenesis and their role in the consequent progression of the CMV life cycle. Our functional analysis of PI(3)P was based on rapid depletion of PI(3)P production at EE membranes using VPS34-IN1, a specific and reversible inhibitor of Vps34 component of class III PI(3)P kinase, and on saturation of PI(3)P by overexpression of recombinant PI(3)P-binding domains (2xFYVE, p40PX). PI(3)P depletion led to rapid reorganization of the Rab11a-dependent pathway of the ERC, and abolished recycling cargo sorting in this pathway as well as membrane flux from EE to the ERC. Despite these alterations and although PI(3)P+ and Vps34+ endosomes build a substantial part of cVAC, the PI(3)P inhibition did not prevent the establishment of infection, rogression through the early phase of infection, and membrane reorganization associated with cVAC development, as analyzed by confocal imaging and Western blot analysis. However, the drastically reduced viral growth under PI(3)P depletion and the significant inhibition of late infection events (DNA replication, late protein expression) together with the reduced release of infectious virions suggest that PI(3)P is essential for entry into the late phase of infection and the final formation of progeny virions.
Jezikengleski
Vrsta recenzijeNepoznato
URN:NBNurn:nbn:hr:184:244380
Datum izlaganja2022-07-26
ProjektŠifra: IP-2019-04-3582
Naziv (hrvatski): Sekundarno omatanje i izlazak beta-herpesvirusa iz stanice
Voditelj: Pero Lučin
Pravna nadležnost: Hrvatska
Financijer: HRZZ
ProjektŠifra: IP-2020-02-1323
Naziv (hrvatski): Stanični sustav Arf GTPaza u patogenezi citomegalovirusne infekcije
Voditelj: Gordana Blagojević Zagorac
Pravna nadležnost: Hrvatska
Financijer: HRZZ
ProjektŠifra: IP-2020-02-2916
Naziv (hrvatski): Reorganizacija sučelja između ranih endosoma i endosomalnog reciklirajućeg odjeljka
Voditelj: Hana Mahmutefendić Lučin
Pravna nadležnost: Hrvatska
Financijer: HRZZ
SkupNaziv: 46th International Herpesvirus Workshop
Lokacija: Virtual meeting, Sjedinjene Američke Države
Datum početka: 2022-07-25
Datum završetka: 2022-07-29
Vrsta izlaganja: Predavanje
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
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Datum i vrijeme pohrane2023-04-05 11:44:12