Milk is a key source of animal protein and essential nutrients, especially for children. Contamination of milk with mycotoxins, toxins produced by molds, can significantly threaten its quality and safety. Mycotoxins, present in food, are a certain risk for humans and animals due to the possibility of causing diseases such as cancer and immunosuppression. Aflatoxin is mainly produced by the molds Aspergillus flavus and Aspergillus parasiticus. Hydroxylated metabolites of aflatoxin B1 and B2, known as M1 and M2, can be found in milk and milk products from livestock that have consumed contaminated feed. The aim of this research is to examine the presence of aflatoxin M1 in milk samples using the AflaSensor nova test. The test enables analysis without complex sample preparation and provides results in ten minutes. The results showed that AflaSensor nova effectively identifies aflatoxin M1 in milk samples, which enables timely action and preservation of high quality dairy products. The obtained results show that the tested commercially available milk and domestic raw milk contain an acceptable amount of aflatoxin M1 and are in accordance with the regulations of the European Union. Only one tested sample of milk is not suitable for consumption by infants considering the prescribed permitted amounts of aflatoxin M1 in milk for infants. This method ensures proper supervision of raw milk, during processing and in the final control of dairy products, which protects the health of consumers and maintains safety on the market.