prikaz prve stranice dokumenta MiRNA-mRNA integrative analysis reveals epigenetically regulated and prognostic miR-103a with a role in migration and invasion of carboplatin-resistant ovarian cancer cells that acquired mesenchymal-like phenotype
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MiRNA-mRNA integrative analysis reveals epigenetically regulated and prognostic miR-103a with a role in migration and invasion of carboplatin-resistant ovarian cancer cells that acquired mesenchymal-like phenotype
Biomedicine & Pharmacotherapy, 166 (2023); 115349. https://doi.org/10.1016/j.biopha.2023.115349
Pernar Kovač, Margareta; Tadić, Vanja; Kralj, Juran; Milković Periša, Marija; Orešković, Slavko; Babić, Ivan; Banović, Vladimir; Zhang, Wei; Culig, Zoran; Brozović, Anamaria

Institucijski repozitorij: Dr Med - digitalni repozitorij Medicinskog fakulteta Sveučilišta u Zagrebu

Citirajte ovaj rad

Pernar Kovač, M., Tadić, V., Kralj, J., Milković Periša, M., Orešković, S., Babić, I. ... Brozović, A. (2023). MiRNA-mRNA integrative analysis reveals epigenetically regulated and prognostic miR-103a with a role in migration and invasion of carboplatin-resistant ovarian cancer cells that acquired mesenchymal-like phenotype. Biomedicine & Pharmacotherapy, 166.. doi: 10.1016/j.biopha.2023.115349

Pernar Kovač, Margareta, et al. "MiRNA-mRNA integrative analysis reveals epigenetically regulated and prognostic miR-103a with a role in migration and invasion of carboplatin-resistant ovarian cancer cells that acquired mesenchymal-like phenotype." Biomedicine & Pharmacotherapy, vol. 166, 2023. https://doi.org/10.1016/j.biopha.2023.115349

Pernar Kovač, Margareta, Vanja Tadić, Juran Kralj, Marija Milković Periša, Slavko Orešković, Ivan Babić, Vladimir Banović, Wei Zhang, Zoran Culig i Anamaria Brozović. "MiRNA-mRNA integrative analysis reveals epigenetically regulated and prognostic miR-103a with a role in migration and invasion of carboplatin-resistant ovarian cancer cells that acquired mesenchymal-like phenotype." Biomedicine & Pharmacotherapy 166 (2023). https://doi.org/10.1016/j.biopha.2023.115349

Pernar Kovač, M., et al. (2023) 'MiRNA-mRNA integrative analysis reveals epigenetically regulated and prognostic miR-103a with a role in migration and invasion of carboplatin-resistant ovarian cancer cells that acquired mesenchymal-like phenotype', Biomedicine & Pharmacotherapy, 166. doi: 10.1016/j.biopha.2023.115349

Pernar Kovač M, Tadić V, Kralj J, Milković Periša M, Orešković S, Babić I, i sur.. MiRNA-mRNA integrative analysis reveals epigenetically regulated and prognostic miR-103a with a role in migration and invasion of carboplatin-resistant ovarian cancer cells that acquired mesenchymal-like phenotype. Biomedicine & Pharmacotherapy [Internet]. 10.2023. [pristupljeno 25.08.2024.];166. doi: 10.1016/j.biopha.2023.115349

M. Pernar Kovač, et al., "MiRNA-mRNA integrative analysis reveals epigenetically regulated and prognostic miR-103a with a role in migration and invasion of carboplatin-resistant ovarian cancer cells that acquired mesenchymal-like phenotype", Biomedicine & Pharmacotherapy, vol. 166, Listopad 2023. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:105:807947. [Citirano: 25.08.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:105:807947
Podaci o radu
Naslov (engleski)MiRNA-mRNA integrative analysis reveals epigenetically regulated and prognostic miR-103a with a role in migration and invasion of carboplatin-resistant ovarian cancer cells that acquired mesenchymal-like phenotype
AutorMargareta Pernar Kovač
AutorVanja Tadić
AutorJuran Kralj
AutorMarija Milković Periša
AutorSlavko Orešković
AutorIvan Babić
AutorVladimir Banović
AutorWei Zhang
AutorZoran Culig
AutorAnamaria Brozović
Autorova ustanovaSveučilište u Zagrebu
Medicinski fakultet
(KATEDRA ZA GINEKOLOGIJU I OPSTETRICIJU)
Znanstveno / umjetničko
područje, polje i grana		BIOMEDICINA I ZDRAVSTVO
Kliničke medicinske znanosti
Ginekologija i opstetricija
Sažetak (engleski)
Background: DNA methylation, histone modifications, and miRNAs affect ovarian cancer (OC) progression and therapy response. Purpose: Identification of epigenetically downregulated miRNAs in drug-resistant OC cell lines with a possible role in drug resistance and/or drug-induced mesenchymal-like phenotype. Methods: MiRNA profiling was performed on parental and carboplatin-resistant OC cells, MES-OV and MES-OV CBP. RT-qPCR validation, epigenetic modulation and other CBP-resistant OC cell lines were used to select miRNAs of interest. The integration of miRNA-predicted target genes and differentially expressed genes (DEGs), pathway and functional analysis were used for forecasting their biological role. Data mining was performed to determine their possible prognostic and predictive values. Results: MiRNA profiling revealed 48 downregulated miRNAs in OC cells whose drug sensitivity and metastatic potential were impacted by epigenetic modulators. Of the fourteen selected, nine were validated as changed, and seven of these restored their expression upon treatment with epigenetic inhibitors. Only three had similar expression patterns in other OC cell lines. MiRNA-mRNA integrative analysis resulted in 56 target DEGs. Pathway analysis revealed that these genes are involved in cell adhesion, migration, and invasion. The functional analysis confirmed the role of miR-103a-3p, miR-17-5p and miR-107 in cell invasion, while data mining showed their prognostic and predictive values. Only miR-103a-3p was epigenetically regulated at the constitutive level. Conclusion: High throughput miRNA and cDNA profiling coupled with pathway analysis and data mining delivered evidence for miRNAs which can be epigenetically regulated in drug-resistant, mesenchymal-like OC cells as possible markers to combat therapy-induced short overall survival and tumor metastatic potential.
Ključne riječi (engleski)
Jezikengleski
Vrsta publikacijeZnanstveni rad - Izvorni znanstveni rad
Status objaveObjavljen
Vrsta recenzijeRecenziran
Verzija publikacijeObjavljena verzija rada (izdavačev PDF)
Naslov časopisaBiomedicine & Pharmacotherapy
Brojčani podacivol. 166, 115349
p-ISSN0753-3322
DOIhttps://doi.org/10.1016/j.biopha.2023.115349
URN:NBNurn:nbn:hr:105:807947
Datum objave publikacije2023-10
URL dokumentahttps://linkinghub.elsevier.com/retrieve/pii/S075333222301140X
Vrsta resursaTekst
Prava pristupaOtvoreni pristup
Uvjeti korištenjaIkona licence Imenovanje-Nekomercijalno-Bez prerada 4.0 međunarodna (CC BY-NC-ND 4.0)
Datum i vrijeme pohrane2024-08-19 08:25:57