Introduction: Although vaccination is one of the most effective public health measures, people increasingly consider vaccination unnecessary and unsafe, refusing or delaying vaccinations for various reasons. This study was designed to examine the attitudes of health professionals about vaccines included in the mandatory vaccine schedule and compare them to the general population, as well as to show differences in attitudes and knowledge between the supporters, opponents and hesitants in vaccination. Methods: The study was conducted over the social networks. The questionnaire was created for the purposes of this study and consisted of 19 questions. Of the 263 respondents included in the study, 68 were men and 195 women. Data was analyzed with descriptive statistics using the statistical program MedCalc. Results: Health professionals showed greater knowledge and more positive attitude about vaccination, significantly more vaccinated their children and were more informed by other health professionals and health institutions. General population is more inclined to believe that vaccines cause autism and burden the immune system of the child, that the vaccine is unnecessary and that they are made for profit of pharmaceutical industry. There is significantly more opponents of vaccination in the general population (16.5%) than with health professionals (2.2%). Opponents are often informed about vaccination through various websites, social networks and forums. Women and older respondents were more often supporters of vaccination, while personal experience and education did not influence on the attitudes towards vaccinations. The biggest concern among vaccines was manifested to a combined vaccine '5in1'. Conclusion: Informing the population by trained health professionals, critical thinking about various unreliable sources of information could favorably influence the change of the current image of vaccination in public.