Professional stress in the work environment is a specific type of stress, whose source is in the work environment itself. An individual assessment and an experience of the objective state or event are affecting at the appearance of stress. Healthcare professionals have a high degree of exposure to stressful situations and the emergence of combustion syndrome due to their great responsibility for human life, health and are exposed to specific stressors (chemical, biological, physical). The aim of the study was to examine the most frequent stress factors on which are exposed nurses and technicians at the United emergency admission unit at the General hospital in Šibenik, and whether there is a difference in stress experience with regard to socio-demographic characteristics (sex, age, total length of work and completed degree of education ). The total number of respondents who participated in the survey was 26 [N = 26]. The general data on participants were collected by the questionnaire of sociodemographic data, the total stress experience was measured by the Stressor Questionnaire at the workplace of hospital healthcare workers (Milošević, 2010). Participants completed the questionnaire at their workplace, and research was a paper-pencil type. United emergency admission unit is one of the most stimulating and most challenging environments in which a nurse is working, but also one of the most stressful jobs of healthcare professionals. The results obtained in this study show that there are related factors in relation to the amount of stress at the work position of nurses / technicians at the United emergency admission unit . The toughest stressor is overwork, exposure to inappropriate public criticism and night work. To women, organization and finances are significantly more stressful than to men. The shift work is significantly more stressful at subjects with 12 years of work experience or more.