Leksell frame-based biopsy and robotic biopsy are two types of stereotactic biopsy that are being used for taking brain tissue for histopathological analysis. With the advancement of technology during time, the integration of robotics in medicine has been growing and its use, especially in performing surgical procedures. Team of physicians from University Hospital Dubrava in cooperation with Faculty of Mechanical Engineering and Naval Architecture, University of Zagreb and Croatian Institute for Brain Research have developed robotic surgical system RONNA (Robotical Neurosurgical Navigation) with the aim of replacing a surgeon performing stereotactic navigation procedures. RONNA is a two-handed robot with high precision optical system and it is being used for performing stereotactic biopsy. Basic idea is to identify the coordinate system of previously recorded head, respectively to determine the target point in relation to external marker on the patients head. Based on radiological images, the robot recognizes features and position of the head in space and with that data performs the procedure. General aim of this study is to show that there is no difference in efficacy between this type of biopsy and classical frame-based biopsy. Objectives of the study are to structure the data about patients into two groups, to test if the control variables depend on qualifying in a specific group (classical or robotical) and to test the independence of specific procedure outcomes on the group. Equal data is collected between patients that underwent frame-based stereotactic biopsy and those who underwent frameless stereotactic biopsy. Control variables whose independence was tested were age, sex, duration of the illness, Karnofsky scale, symptoms and the neurosurgeon. Variables post operation scan, complications and clinical status, histopathological diagnosis and length of hospitalization were used as procedure outcomes. Results showed that two groups do not differ by control variables and that there is no statistically significant difference in outcomes in these two groups. By tested parameters, robotic biopsy is equally successful as Leksell frame-based biopsy.