With the advancement of technology in dental medicine, nurses must constantly improve. Thus, with the advent of new technology, plasma enriched with growth factors is also used at the Department of Oral Surgery. Plasma is obtained from the patient's blood, which is performed using the venipuncture technique just before the procedure in the oral cavity. Growth factors are 100% autologous material obtained from the patient's blood, to which the patient is not allergic. It can also be mixed with artificial bone and is used to regenerate soft and hard dental tissues. It is also used after tooth extraction, alveotomy, cystectomy, lifting the bottom of the maxillary sinus, implantation of dental implants. The nurse must possess knowledge and skills, must undergo additional training to successfully prepare plasma enriched with growth factors. After venipuncture, the blood is centrifuged, then pipetted, activated, and placed in plasmatherm. The obtained material is transferred to a sterile table under aseptic conditions and implanted in a defect in the patient's mouth. It serves for faster creation of soft and hard dental tissues, and for the patient to have the best possible postoperative recovery. The protocol involves the extraction of venous blood from the patient and centrifugation, which achieves the separation of erythrocytes and leukocytes and produces plasma rich in growth factors. It is crucial that the dental assistant is familiar with the venipuncture technique, which is presented in more detail in the paper.